Determination of Phenolic Compounds in Pinus eldarica by HPLC

Authors

  • B Larijani Tehran University of Medical Sciences
  • E Taheri Tehran University of Medical Sciences
  • F Sharifi Tehran University of Medical Sciences
  • H Fallah Huseini Research Center, Institute of Medicinal Plants, ACECR
  • M Mirarefin University of California
  • M Sadeghi Afjeh Tehran University of Medical Sciences
  • S Saeednia Tehran University of Medical Sciences
  • Y Tajalizadekhoob Tehran University of Medical Sciences
Abstract:

Background: The antioxidant components have been identified in some pine species. Antioxidant properties of proantocyanidins reduce free radicals induced by DNA fragmentation and lipid proxidation and also proanthocyanidines could curb lipid peroxidation. Objective: In this study, we analyzed different parts of Pinus eldarica (bark, seed and needle) and assessed their antioxidant contents. Methods: Pine specimens were collected from four different geographic locations in Tehran. The HPLC method (UV detector, C18 reverse phase column, 4.6 mm (25 cm, and water/ H3PO4/ methanol/ acetonitril as eluant) were employed for evaluating total polyphenols. The wavelength for detection of polyphenolic compounds was 280 nm in this study. Results: The highest range of total polyphenols was detected in the bark of this pine, specially reported a considerable amount of tyrosol in Pinus eldarica. Tyrosol stimulated resistance to oxidative stress and also has anti aging effect. Conclusion: The high amount of total phenolic compounds in P. eldarica bark might be attractive for future research considering its health benefits.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

determination of phenolic compounds in pinus eldarica by hplc

background: the antioxidant components have been identified in some pine species. antioxidant properties of proantocyanidins reduce free radicals induced by dna fragmentation and lipid proxidation and also proanthocyanidines could curb lipid peroxidation. objective: in this study, we analyzed different parts of pinus eldarica (bark, seed and needle) and assessed their antioxidant contents. meth...

full text

Phytochemical analysis of Pinus eldarica bark

Bark extract of Pinus pinaster contains numerous phenolic compounds such as catechins, taxifolin, and phenolic acids. These compounds have received considerable attentions because of their anti-inflammatory, antimutagenic, anticarcinogenic, antimetastatic and high antioxidant activities. Although P. pinaster bark has been intensely investigated in the past; there is comparably less information ...

full text

Spectrophotometric Determination of Phenolic Compounds in Propolis

Three spectrophotometric methods were evaluated in order to determine total phenolic compounds content in propolis from Tucumán, Argentina; applying the Folin-Ciocalteau, Prussian blue and ophenanthroline methods. The Prussian blue method was the most sensitive one although it was also unstable. The o-phenanthroline method may be more reliable and proved to be quite sensitive and stable. FolinC...

full text

The Evaluation of Antioxidant Activities and Phenolic Compounds in Leaves and Inflorescence of Artemisia dracunculus L. by HPLC

Background: Artemisia dracunculus L. belongs to the Anthemideae tribe of Asteraceae family. This plant has been used in traditional folk medicine. Also, it's anti-bacterial, anti-fungal, antioxidant, anti-inflammatory and hepatoprotective activity has been proved. Objective: The purpose of this study was to evaluate the antioxidant activity of leaves and inflorescence of A. dracunculus and als...

full text

Fingerprint analysis and simultaneous determination of phenolic compounds in extracts of Curculiginis Rhizoma by HPLC-diode array detector.

Curculiginis Rhizoma (Curculigo orchioides GAERTN.) is a well-known Chinese herbal medicine, as well as an important Rasayana drug in India. Current criteria of quality control on this herb are to quantitatively analyze single compound curculigoside, which fail to comprehensively evaluate quality of this herb. In this paper, a simple and reliable HPLC coupled with diode array detector (DAD) met...

full text

Simultaneous determination of 14 phenolic compounds in grape canes by HPLC-DAD-UV using wavelength switching detection.

The paper described a novel chromatographic method for the simultaneous determination of phenolic compounds such as gallic, protocatechuic, vanillic, caffeic, syringic, p-coumaric and salicylic acid, (+)-catechin, (‒)-epicatechin, rutin, morin, quercetin, coumarin and trans-resveratrol at their maximum absorbance wavelengths (MAW) employing reverse-phase high performance liquid chromatography c...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 1  issue 49

pages  22- 33

publication date 2014-03

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023